1Z5V

Crystal structure of human gamma-tubulin bound to GTPgammaS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2004-07-10
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	52.427, 76.325, 65.197
Unit cell angles	90.00, 101.93, 90.00

Refinement procedure

Resolution	29.040 - 2.710
R-factor	0.237
Rwork	0.237
R-free	0.29300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1sa0 chain B truncated to poly-ala
RMSD bond length	0.008
RMSD bond angle	1.400
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.870
High resolution limit [Å]	2.700	2.700
Rmerge	0.061	0.380
Number of reflections	12530
<I/σ(I)>	13.5	3
Completeness [%]	96.1	65.8
Redundancy	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8.2	277	Tris, KCL, PEG6000, pH 8.2, VAPOR DIFFUSION, temperature 277K
1	VAPOR DIFFUSION	8.2	277	Tris, KCL, PEG6000, pH 8.2, VAPOR DIFFUSION, temperature 277K