1Z5R
Crystal Structure of Activated Porcine Pancreatic Carboxypeptidase B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2002-03-11 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.009 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 66.815, 96.055, 135.821 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.400 |
| R-factor | 0.177 |
| Rwork | 0.175 |
| R-free | 0.20900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1NAS catalytic domain |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.860 |
| Data reduction software | X-GEN |
| Data scaling software | X-GEN |
| Phasing software | MOLREP |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.490 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Number of reflections | 161497 | |
| <I/σ(I)> | 19.4 | 2 |
| Completeness [%] | 93.9 | 72.7 |
| Redundancy | 3.5 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | sodium chloride, Tris, MGTA, sodium cacodylate, PEG 8000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
