1Z5P
Crystal structure of MTA/AdoHcy nucleosidase with a ligand-free purine binding site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 2 2 2 |
| Unit cell lengths | 70.010, 127.520, 69.590 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.270 - 2.000 |
| R-factor | 0.206 |
| Rwork | 0.206 |
| R-free | 0.23400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jys |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.700 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | CNS (1.1) |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.270 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.056 | 0.346 |
| Number of reflections | 21178 | |
| Completeness [%] | 97.4 | |
| Redundancy | 9.8 | 9.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | ammonium sulfate, PEG 2000 MME, sodium HEPES, isopropanol, QX-10000024, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
