1Z52
Proaerolysin Mutant W373L
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-ID-B |
| Synchrotron site | APS |
| Beamline | 14-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-11 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.1170 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 70.711, 91.141, 168.215 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.380 |
| Rwork | 0.250 |
| R-free | 0.30140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.864 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.470 |
| High resolution limit [Å] | 2.380 | 2.380 |
| Number of reflections | 42497 | |
| Completeness [%] | 96.2 | 73.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 295.15 | PEG 4000, sodium acetate, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 295.15K |
