1Z50

Parainfluenza Virus 5 (SV5) Hemagglutinin-Neuraminidase (HN) with ligand DANA (soaked with sialic acid, pH 8.0)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 32-ID
Synchrotron site	APS
Beamline	32-ID
Temperature [K]	100
Detector technology	CCD
Detector	MARRESEARCH
Wavelength(s)	0.9918
Spacegroup name	P 61 2 2
Unit cell lengths	137.661, 137.661, 184.917
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.800
R-factor	0.2
Rwork	0.199
R-free	0.22400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1e8t
RMSD bond length	0.007
RMSD bond angle	1.451
Data scaling software	SCALEPACK
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.900
High resolution limit [Å]	2.800	6.030	2.800
Rmerge	0.119	0.062	0.394
Number of reflections	26160
Completeness [%]	100.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	hanging drop	8	291	PEG 2000 MME, NaCl, Tris, pH 8.0, hanging drop, temperature 291K