1Z4Y

Parainfluenza Virus 5 (SV5) Hemagglutinin-Neuraminidase (HN) (pH 8.0)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 32-ID
Synchrotron site	APS
Beamline	32-ID
Temperature [K]	100
Detector technology	CCD
Detector	MARRESEARCH
Wavelength(s)	1.000
Spacegroup name	P 61 2 2
Unit cell lengths	137.600, 137.600, 184.950
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.600
R-factor	0.217
Rwork	0.216
R-free	0.23500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1e8t
RMSD bond length	0.007
RMSD bond angle	1.473
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.690
High resolution limit [Å]	2.600	5.600	2.600
Rmerge	0.098	0.049	0.427
Number of reflections	31340
Completeness [%]	96.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	hanging drop	8	291	PEG 2000 MME, NaCl, Tris, pH 8.0, hanging drop, temperature 291K