1Z4W

Parainfluenza Virus 5 (SV5) Hemagglutinin-Neuraminidase (HN) with ligand DANA (soaked with DANA, pH8.0)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 32-ID
Synchrotron site	APS
Beamline	32-ID
Temperature [K]	100
Detector technology	CCD
Detector	MARRESEARCH
Wavelength(s)	0.9999
Spacegroup name	P 61 2 2
Unit cell lengths	137.976, 137.976, 185.041
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.700
R-factor	0.202
Rwork	0.198
R-free	0.22200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1e8t
RMSD bond length	0.007
RMSD bond angle	1.420
Data scaling software	SCALEPACK
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.800
High resolution limit [Å]	2.700	5.810	2.700
Rmerge	0.083	0.050	0.346
Number of reflections	27908
Completeness [%]	95.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	hanging drop	8	291	PEG 2000 MME, NaCl, Tris, pH 8.0, hanging drop, temperature 291K