1Z4O
Structure of beta-phosphoglucomutase with inhibitor bound alpha-galactose 1-phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-01-01 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.069, 36.351, 104.883 |
| Unit cell angles | 90.00, 101.77, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| R-factor | 0.205 |
| Rwork | 0.199 |
| R-free | 0.25400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1O08.pdb |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.800 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.076 | 0.394 |
| Number of reflections | 32586 | |
| <I/σ(I)> | 14.5 | 4.2 |
| Completeness [%] | 99.4 | 99.2 |
| Redundancy | 3 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 200 mM sodium acetate, 30% PEG 4000, 10 mM magnesium dicloride, 50 mM alpha-galactose 1-phosphate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 18K |
