1Z47
Structure of the ATPase subunit CysA of the putative sulfate ATP-binding cassette (ABC) transporter from Alicyclobacillus acidocaldarius
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-07-11 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9393 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 154.810, 56.310, 91.620 |
| Unit cell angles | 90.00, 91.72, 90.00 |
Refinement procedure
| Resolution | 19.970 - 1.900 |
| R-factor | 0.2251 |
| Rwork | 0.225 |
| R-free | 0.27700 |
| Structure solution method | MAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 58181 | |
| <I/σ(I)> | 20.1 | 5.3 |
| Completeness [%] | 92.8 | 69 |
| Redundancy | 3.6 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 291 | PEG 4000, iospropanol, sodiumcitrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
