1Z43
Crystal structure of 7S.S SRP RNA of M. jannaschii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Wavelength(s) | 1.081 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 62.110, 62.110, 247.520 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.600 |
| R-factor | 0.2552 |
| Rwork | 0.253 |
| R-free | 0.29616 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lng |
| RMSD bond length | 0.020 |
| RMSD bond angle | 3.226 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | CNS |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.700 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 15645 | |
| Completeness [%] | 98.9 | 98.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | PEG 400, Tris buffer, sodium citrate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 400 | ||
| 2 | 1 | 1 | Tris buffer | ||
| 3 | 1 | 1 | sodium citrate | ||
| 4 | 1 | 1 | H2O | ||
| 5 | 1 | 2 | PEG 400 | ||
| 6 | 1 | 2 | Tris buffer | ||
| 7 | 1 | 2 | sodium citrate | ||
| 8 | 1 | 2 | H2O |
