1Z40
AMA1 from Plasmodium falciparum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-01-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.100 |
| Spacegroup name | P 31 |
| Unit cell lengths | 54.124, 54.124, 214.144 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.220 - 1.901 |
| R-factor | 0.19461 |
| Rwork | 0.192 |
| R-free | 0.23560 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.436 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.220 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 54498 | |
| <I/σ(I)> | 30 | 11 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 9.5 | 11 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 295 | PEG 3350, MES, manganese chloride, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
