1Z3V
Structure of Phanerochaete chrysosporium cellobiohydrolase Cel7D (CBH58) in complex with lactose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-12-11 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9322 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 86.947, 46.700, 99.124 |
| Unit cell angles | 90.00, 102.77, 90.00 |
Refinement procedure
| Resolution | 39.220 - 1.610 |
| R-factor | 0.1605 |
| Rwork | 0.158 |
| R-free | 0.20197 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gpi |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.511 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.072 | 0.238 |
| Number of reflections | 50272 | |
| <I/σ(I)> | 14.8 | 8.1 |
| Completeness [%] | 99.3 | 99.6 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 294 | TRIS-HCl, calcium chloride, PEG 5000, glycerol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
