1Z3T
Structure of Phanerochaete chrysosporium cellobiohydrolase Cel7D (CBH58) in complex with cellobiose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-02-07 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9370 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 87.414, 46.842, 99.488 |
| Unit cell angles | 90.00, 102.99, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.700 |
| R-factor | 0.16125 |
| Rwork | 0.159 |
| R-free | 0.19866 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gpi |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.062 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.098 | 0.321 |
| Number of reflections | 43328 | |
| <I/σ(I)> | 11 | 5.8 |
| Completeness [%] | 99.6 | 93.1 |
| Redundancy | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 294 | TRIS-HCl, calcium chloride, PEG 5000, glycerol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
