1Z3Q
Resolution of the structure of the allergenic and antifungal banana fruit thaumatin-like protein at 1.7A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-02-02 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.844, 52.839, 80.606 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.700 |
| R-factor | 0.14426 |
| Rwork | 0.142 |
| R-free | 0.17712 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1thv |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.354 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.200 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 20095 | |
| <I/σ(I)> | 17.6 | 9.4 |
| Completeness [%] | 96.9 | 94.9 |
| Redundancy | 4.6 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | PEG 4000, Ammonium Sulfate, Sodium Acatate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
