1Z3I
Structure of the SWI2/SNF2 chromatin remodeling domain of eukaryotic Rad54
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-06-01 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 205.046, 205.046, 189.958 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 3.000 |
| R-factor | 0.19957 |
| Rwork | 0.198 |
| R-free | 0.23430 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.236 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 31235 | |
| Completeness [%] | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 298 | Ammonium Sulfate, Malonate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K, pH 6.00 | |
| 1 | 6 | 298 | Ammonium Sulfate, Malonate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K, pH 6.00 | |
| 1 | 6 | 298 | Ammonium Sulfate, Malonate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K, pH 6.00 |
