1Z2Z
Crystal Structure of the Putative tRNA pseudouridine synthase D (TruD) from Methanosarcina mazei, Northeast Structural Genomics Target MaR1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-02-06 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97931 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.562, 132.531, 66.779 |
| Unit cell angles | 90.00, 116.98, 90.00 |
Refinement procedure
| Resolution | 29.030 - 2.600 |
| R-factor | 0.233 |
| Rwork | 0.233 |
| R-free | 0.29600 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SnB |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.058 | 0.252 |
| Number of reflections | 58176 | |
| <I/σ(I)> | 23.62 | 5.4 |
| Completeness [%] | 99.2 | 99.1 |
| Redundancy | 4.3 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 100 mM sodium cacodylate acid (pH 6.5), 16% PEG 8k, 200 mM magnesium acetate, and 5 mM DTT. , VAPOR DIFFUSION, HANGING DROP, temperature 293K |
