1Z2V
Crystal Structure of Glu60 deletion Mutant of Human Acidic Fibroblast Growth Factor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 103 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-04-20 |
| Detector | RIGAKU RAXIS IIC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 73.239, 97.984, 109.269 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.840 - 1.900 |
| Rwork | 0.186 |
| R-free | 0.22100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jqz |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.800 | 1.960 |
| High resolution limit [Å] | 1.890 | 1.890 |
| Rmerge | 0.052 | 0.277 |
| Number of reflections | 26149 | |
| <I/σ(I)> | 28.86 | 3.1 |
| Completeness [%] | 87.7 | 70.5 |
| Redundancy | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298.15 | PEG400, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.15K |
