1Z1S
Crystal Structure of Putative Isomerase PA3332 from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-03-04 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 54.713, 54.713, 92.121 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.400 - 1.490 |
| R-factor | 0.17152 |
| Rwork | 0.168 |
| R-free | 0.20492 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.423 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0000) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.400 | 1.540 |
| High resolution limit [Å] | 1.490 | 1.490 |
| Rmerge | 0.066 | 0.385 |
| Number of reflections | 25574 | |
| <I/σ(I)> | 10 | 2.1 |
| Completeness [%] | 95.7 | 66.4 |
| Redundancy | 16.1 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 295 | BIS-TRIS, Magnesium acetate, PEG3350, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
