1Z1F
Crystal structure of stilbene synthase from Arachis hypogaea (resveratrol-bound form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL44B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-10-19 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 100.746, 100.746, 74.080 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.590 - 2.900 |
| R-factor | 0.192 |
| Rwork | 0.192 |
| R-free | 0.25200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.000 | 3.060 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Number of reflections | 9234 | |
| <I/σ(I)> | 9.6 | 3.4 |
| Completeness [%] | 93.1 | 84.7 |
| Redundancy | 7.2 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | 100mM sodium citrate pH 4.6, 150mM ammonium sulfate, 16%(w/v) polyethylene glycol 6000, 10%(v/v) glycerol, 5mM resveratrol, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
