1Z18

Crystal structure analysis of periplasmic Leu/Ile/Val-binding protein with bound valine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-04-05
Detector	MACSCIENCE
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	58.420, 70.340, 82.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.210 - 2.100
Rwork	0.216
R-free	0.26200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	liv-leu complex without bound leu and solvent pdb entry 1Z16
RMSD bond length	0.011
RMSD bond angle	1.600
Data reduction software	MACSCIENCE
Data scaling software	SCALEPACK
Phasing software	EPMR ((KISSINGER)
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.200
High resolution limit [Å]	2.100	2.100
Rmerge	0.129	0.242
Number of reflections	18896
<I/σ(I)>	5.4	2.2
Completeness [%]	92.0	85
Redundancy	3	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	25% (v/v) PEG-400, 0.1 M succinic acid, 50 mM CdSo4 and 50 mM Na-cacodylate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K