1Z15

Crystal structure analysis of periplasmic Leu/Ile/Val-binding protein in superopen form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	293
Detector technology	AREA DETECTOR
Collection date	1990-01-10
Detector	SDMS
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	57.210, 65.290, 109.610
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 1.700
Rwork	0.185
R-free	0.23700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2liv
RMSD bond length	0.012
RMSD bond angle	1.500
Data reduction software	SDMS
Data scaling software	SDMS
Phasing software	MERLOT
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.750
High resolution limit [Å]	1.700	1.700
Rmerge	0.069	0.277
Number of reflections	44773
<I/σ(I)>	9.8	1.9
Completeness [%]	96.0	89
Redundancy	4	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICRODIALYSIS	7.3	293	12.5% PEG 6000, potassium chloride, sodium choloride, sodium azide, potassium acetate, sodium citrate, pH 7.3, MICRODIALYSIS, temperature 293K