1Z14

Structural Determinants of Tissue Tropism and In Vivo Pathogenicity for the Parvovirus Minute Virus of Mice

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	295
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Wavelength(s)	0.870
Spacegroup name	C 1 2 1
Unit cell lengths	448.700, 416.500, 306.100
Unit cell angles	90.00, 95.90, 90.00

Refinement procedure

Resolution	20.000 - 3.250
Rwork	0.298
R-free	0.30600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	canine parvovirus model
RMSD bond length	0.010
RMSD bond angle	1.583
Data scaling software	SCALEPACK
Phasing software	GLRF
Refinement software	CNS (1.0)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	3.250
Rmerge	0.162
Number of reflections	517599
Completeness [%]	64.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5		10mM Tris-HCL, 8 mM CaCl2, PEG8000 and virus 10 mgs/ml, pH 7.5, VAPOR DIFFUSION, HANGING DROP
1	VAPOR DIFFUSION, HANGING DROP	7.5		10mM Tris-HCL, 8 mM CaCl2, PEG8000 and virus 10 mgs/ml, pH 7.5, VAPOR DIFFUSION, HANGING DROP