1Z12

Crystal Structure of Bovine Low Molecular Weight PTPase Complexed with Vanadate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	1995-01-01
Detector	RIGAKU RAXIS II
Wavelength(s)	1.54
Spacegroup name	C 1 2 1
Unit cell lengths	95.300, 43.400, 41.200
Unit cell angles	90.00, 113.40, 90.00

Refinement procedure

Resolution	20.000 - 2.200
R-factor	0.173
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1dg9
RMSD bond length	0.010
RMSD bond angle	2.320
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	TNT
Refinement software	TNT

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.200
Number of reflections	6260
Completeness [%]	80.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	295	PEG 4000, HEPES, Isopropanol, Na2VO4, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K
1	VAPOR DIFFUSION, SITTING DROP	7.5	295	PEG 4000, HEPES, Isopropanol, Na2VO4, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K
1	VAPOR DIFFUSION, SITTING DROP	7.5	295	PEG 4000, HEPES, Isopropanol, Na2VO4, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K