1Z0N
the glycogen-binding domain of the AMP-activated protein kinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-ID-B |
| Synchrotron site | APS |
| Beamline | 14-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-10 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 1 |
| Unit cell lengths | 43.750, 45.250, 50.600 |
| Unit cell angles | 71.90, 69.70, 65.40 |
Refinement procedure
| Resolution | 23.270 - 1.490 |
| R-factor | 0.184 |
| Rwork | 0.184 |
| R-free | 0.21256 |
| Structure solution method | MIR |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.666 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.000 | 1.510 |
| High resolution limit [Å] | 1.480 | 1.480 |
| Rmerge | 0.049 | 0.332 |
| Number of reflections | 23738 | |
| Completeness [%] | 95.9 | 91.4 |
| Redundancy | 2.4 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 299 | PEG, monomethyl ether 5000, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 299K |
