1YZV

HYPOTHETICAL PROTEIN FROM TRYPANOSOMA CRUZI

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	CCD
Collection date	2004-11-07
Detector	ADSC QUANTUM 210
Wavelength(s)	1.00000
Spacegroup name	P 4 3 2
Unit cell lengths	121.142, 121.142, 121.142
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	18.470 - 2.001
Rwork	0.157
R-free	0.18680
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.019
RMSD bond angle	1.617
Data reduction software	MOSFLM
Phasing software	EPMR
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall
Low resolution limit [Å]	18.470
High resolution limit [Å]	2.000
Rmerge	0.183
Number of reflections	21028
<I/σ(I)>	12
Completeness [%]	99.0
Redundancy	9.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	290	0.4 ul protein 19 mg/ml 0.4 ul crystallization buffer 20% PEG 4000, 100 mM CaCl2, 100 mM HEPES pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 290K