1YZH
Crystal Structure of the Conserved Hypothetical Protein, Methyltransferase from Streptococcus pneumoniae TIGR4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-02-22 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 165.613, 41.253, 78.658 |
| Unit cell angles | 90.00, 111.87, 90.00 |
Refinement procedure
| Resolution | 36.500 - 2.020 |
| R-factor | 0.18051 |
| Rwork | 0.179 |
| R-free | 0.20798 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.280 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0000) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.500 | 2.090 |
| High resolution limit [Å] | 2.020 | 2.020 |
| Rmerge | 0.070 | 0.194 |
| Number of reflections | 31802 | |
| <I/σ(I)> | 8.5 | 3 |
| Completeness [%] | 96.8 | 74.5 |
| Redundancy | 3.2 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 289 | Citrate, PEG3000, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
