1YZ9
Crystal structure of RNase III mutant E110Q from Aquifex aeolicus complexed with double stranded RNA at 2.1-Angstrom Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-08-09 |
| Detector | BRUKER PROTEUM 300 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.586, 118.362, 61.900 |
| Unit cell angles | 90.00, 99.45, 90.00 |
Refinement procedure
| Resolution | 29.880 - 2.100 |
| R-factor | 0.21 |
| Rwork | 0.208 |
| R-free | 0.23800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1i4s |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.100 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.880 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.084 | 0.365 |
| Number of reflections | 42826 | |
| <I/σ(I)> | 14.5 | 2.56 |
| Completeness [%] | 85.3 | 50.1 |
| Redundancy | 4.4 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 292 | Ammonium sulfate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Ammonium sulfate | ||
| 2 | 1 | 1 | H2O | ||
| 3 | 1 | 2 | Ammonium sulfate | ||
| 4 | 1 | 2 | H2O |
