1YZ5

The crystal structure of 14-3-3-sigma at 2.8 angstrom resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MPG/DESY, HAMBURG BEAMLINE BW6
Synchrotron site	MPG/DESY, HAMBURG
Beamline	BW6
Temperature [K]	90
Detector technology	CCD
Collection date	2004-06-17
Detector	MARRESEARCH
Wavelength(s)	1.05
Spacegroup name	P 21 21 21
Unit cell lengths	71.650, 80.850, 99.130
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.800
R-factor	0.22293
Rwork	0.219
R-free	0.29158
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	: 1QJB
RMSD bond length	0.008
RMSD bond angle	1.232
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	63.250	2.800
High resolution limit [Å]	2.600	2.700
Number of reflections	19766
Completeness [%]	96.0	98.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	278	40% PEG 4000, 0.6M ammonium sulfate, 0.1M Tris/HCl, pH 9, VAPOR DIFFUSION, SITTING DROP, temperature 278K