1YYV
Putative transcriptional regulator ytfH from Salmonella typhimurium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-02-03 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 79.285, 79.285, 105.115 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.350 |
| R-factor | 0.1998 |
| Rwork | 0.201 |
| R-free | 0.24390 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.654 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.410 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.136 | 0.894 |
| Number of reflections | 14468 | |
| <I/σ(I)> | 41.2 | 4.1 |
| Completeness [%] | 99.4 | 99.6 |
| Redundancy | 22.7 | 18.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 293 | 10% PEG 8000, 0.2 M NaCl, sodium/potassium phosphate buffer, pH 6.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
