1YYP
Crystal structure of cytomegalovirus UL44 bound to C-terminal peptide from CMV UL54
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 113 |
Detector technology | CCD |
Collection date | 2003-08-09 |
Detector | SBC-2 |
Wavelength(s) | 0.9793, 0.9794, 1.008, 0.9537 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 91.787, 127.592, 65.975 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.500 |
R-factor | 0.197 |
Rwork | 0.195 |
R-free | 0.22700 |
Structure solution method | MAD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.880 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE (2.02) |
Refinement software | REFMAC (5.1.19) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.610 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.058 | 0.203 |
Number of reflections | 13802 | |
<I/σ(I)> | 44.8 | 13.7 |
Completeness [%] | 99.9 | 100 |
Redundancy | 14.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 295 | 2 M ammonium sulfate, 100 mM phosphate-citrate, and 10 mM DTT , pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 295K |