1YY7
Crystal structure of stringent starvation protein A (SspA), an RNA polymerase-associated transcription factor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9B |
| Synchrotron site | NSLS |
| Beamline | X9B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-02-19 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.06129 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 100.412, 100.412, 181.890 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.460 - 2.020 |
| R-factor | 0.181 |
| Rwork | 0.179 |
| R-free | 0.21500 |
| Structure solution method | MIR |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE (2.01) |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.090 |
| High resolution limit [Å] | 2.020 | 2.020 |
| Rmerge | 0.108 | 0.519 |
| Number of reflections | 36005 | |
| <I/σ(I)> | 16.12 | 2.83 |
| Completeness [%] | 100.0 | 97.4 |
| Redundancy | 6.97 | 5.61 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | diammonium hydrogen citrate, PEG 3350, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
