1YXI

R-State AMP Complex Reveals Initial Steps of the Quaternary Transition of Fructose-1,6-bisphosphatase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	110
Detector technology	IMAGE PLATE
Collection date	2001-02-21
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	I 2 2 2
Unit cell lengths	52.790, 82.800, 165.510
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 2.000
R-factor	0.1991
Rwork	0.199
R-free	0.24200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1cnq
RMSD bond length	0.006
RMSD bond angle	1.400
Data reduction software	TRUNCATE
Data scaling software	CCP4 ((TRUNCATE))
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.910	2.070
High resolution limit [Å]	2.000	2.000
Number of reflections	23957
Completeness [%]	99.6	96.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	293	Leu54 FBPase (10mg/ml), Hepes (25 mM, pH 7.4), MgCl2 (5 mM), F16P2 (5 mM) combined with precipitant solution [Hepes (100mM, pH7.4), PEG 3350 (8% w/v), glycerol (27% v/v), t-butanol (5% v/v)], VAPOR DIFFUSION, HANGING DROP, temperature 293K