1YXC
Structure of E. coli dihydrodipicolinate synthase to 1.9 A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-08-31 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 120.869, 120.869, 110.194 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.560 - 1.900 |
| R-factor | 0.17371 |
| Rwork | 0.172 |
| R-free | 0.21145 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dhp |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.570 |
| Data scaling software | d*TREK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.570 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.089 | 0.358 |
| Number of reflections | 73225 | |
| <I/σ(I)> | 9.7 | 3.5 |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 4.3 | 4.17 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 10 | 276 | 1.8M potassium phosphate, pH 10, VAPOR DIFFUSION, HANGING DROP, temperature 276K |
