1YWP
Phospholipase Cgamma1 SH3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-09 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.009 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 28.967, 31.280, 29.778 |
| Unit cell angles | 90.00, 97.09, 90.00 |
Refinement procedure
| Resolution | 29.490 - 1.600 |
| R-factor | 0.14457 |
| Rwork | 0.142 |
| R-free | 0.18774 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1oeb |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.677 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.037 | 0.168 |
| Number of reflections | 6979 | |
| <I/σ(I)> | 42.2 | 10.5 |
| Completeness [%] | 97.5 | 94.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 298 | PEG 4000, sodium acetate, Tris-HCl, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
