1YWM

Crystal structure of the N-terminal domain of group B Streptococcus alpha C protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	77
Detector technology	CCD
Collection date	2000-12-15
Detector	SBC-2
Spacegroup name	P 61 2 2
Unit cell lengths	56.464, 56.464, 271.617
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.530 - 1.860
R-factor	0.193
Rwork	0.188
R-free	0.23300
Structure solution method	SAD
Starting model (for MR)	ALPHA C SELENOMETHIONYL MUTANT (2 SELENIUM ATOMS)
RMSD bond length	0.015
RMSD bond angle	1.406
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	EPMR
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.930
High resolution limit [Å]	1.860	1.860
Number of reflections	19465
<I/σ(I)>	29.88	4.69
Completeness [%]	95.1	71.7
Redundancy	14

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	294	sodium acetate, PEG4000, DTT, pH 4.60, VAPOR DIFFUSION, HANGING DROP, temperature 294K
1	VAPOR DIFFUSION, HANGING DROP	4.6	294	sodium acetate, PEG4000, DTT, pH 4.60, VAPOR DIFFUSION, HANGING DROP, temperature 294K
1	VAPOR DIFFUSION, HANGING DROP	4.6	294	sodium acetate, PEG4000, DTT, pH 4.60, VAPOR DIFFUSION, HANGING DROP, temperature 294K