1YW6
Crystal Structure of Succinylglutamate Desuccinylase from Escherichia coli, Northeast Structural Genomics Target ET72.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-01-11 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97926, 0.97949, 0.94644 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 146.804, 146.804, 209.449 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.980 - 3.100 |
| R-factor | 0.227 |
| Rwork | 0.227 |
| R-free | 0.28900 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.210 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.113 | 0.776 |
| Number of reflections | 39469 | |
| <I/σ(I)> | 21.25 | 3.4 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 10.1 | 10.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 273 | Tris-HCl (pH 8.0), 1.5 M magnesium sulfate, and DTT., VAPOR DIFFUSION, HANGING DROP, temperature 273K |
