1YW4
Crystal Structure of the Succinylglutamate Desuccinylase from Chromobacterium violaceum, Northeast Structural Genomics Target CvR22.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-02-05 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.950, 39.435, 131.585 |
| Unit cell angles | 90.00, 94.74, 90.00 |
Refinement procedure
| Resolution | 29.950 - 2.000 |
| R-factor | 0.221 |
| Rwork | 0.221 |
| R-free | 0.26200 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SnB |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.062 | 0.255 |
| Number of reflections | 83796 | |
| <I/σ(I)> | 19.7 | 5 |
| Completeness [%] | 98.2 | 96.3 |
| Redundancy | 4 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 100 mM MES (pH 6.0), 18% PEG 2K, 100 mM magnesium chloride, and 5 DTT. , VAPOR DIFFUSION, SITTING DROP, temperature 293K |
