1YW0

Crystal structure of the tryptophan 2,3-dioxygenase from Xanthomonas campestris. Northeast Structural Genomics Target XcR13.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2004-12-04
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.96860
Spacegroup name	P 21 21 21
Unit cell lengths	89.701, 110.223, 149.398
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.320 - 2.700
R-factor	0.25
Rwork	0.250
R-free	0.29400
Structure solution method	SAD
RMSD bond length	0.011
RMSD bond angle	1.600
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELXS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.116	0.740
Number of reflections	71669
Completeness [%]		82.3
Redundancy	4	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291	13-20% PEG 3350, 0.1-0.2 M magnesium chloride, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K