1YVO
hypothetical acetyltransferase from P.aeruginosa PA01
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 150 |
| Detector technology | CCD |
| Collection date | 2005-02-13 |
| Detector | SBC-3 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.015, 84.007, 85.903 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 60.080 - 1.900 |
| R-factor | 0.18681 |
| Rwork | 0.186 |
| R-free | 0.20807 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.170 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.080 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.487 | |
| Number of reflections | 28007 | |
| <I/σ(I)> | 11 | 2 |
| Completeness [%] | 97.9 | 99.3 |
| Redundancy | 4.6 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | 0.4M Ammonium Chloride, 25% PEG3350, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
