1YVN

THE YEAST ACTIN VAL 159 ASN MUTANT COMPLEX WITH HUMAN GELSOLIN SEGMENT 1.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X9B
Synchrotron site	NSLS
Beamline	X9B
Temperature [K]	105
Detector technology	CCD
Collection date	1999-02
Detector	ADSC QUANTUM 4
Spacegroup name	C 1 2 1
Unit cell lengths	176.098, 68.077, 54.691
Unit cell angles	90.00, 102.24, 90.00

Refinement procedure

Resolution	25.000 - 2.100
Rwork	0.187
R-free	0.23500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1yag
RMSD bond length	0.010
RMSD bond angle	1.404
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall
Low resolution limit [Å]	25.000
High resolution limit [Å]	2.100
Rmerge	0.057
Number of reflections	33130
Completeness [%]	86.8
Redundancy	2.95

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8		0.1 M HEPES, 1.60 M (NH4)2SO4, 2 MM MG-ATP, PH=8.0.