1YVM
E. coli Methionine Aminopeptidase in complex with thiabendazole
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 103 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-10-21 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.910, 66.130, 48.530 |
| Unit cell angles | 90.00, 111.25, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.600 |
| R-factor | 0.1699 |
| Rwork | 0.168 |
| R-free | 0.23310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2mat |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.027 |
| Data reduction software | CrystalClear ((MSC/RIGAKU)) |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 29881 | |
| <I/σ(I)> | 14.8 | 2.9 |
| Completeness [%] | 98.2 | 75.8 |
| Redundancy | 3.4 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.1 | 293 | PEG 4000, cobalt chloride, hepes, methionine, sodium chloride, potassium chloride, pH 7.1, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
