1YVI

X-RAY STRUCTURE OF PUTATIVE HISTIDINE-CONTAINING PHOSPHOTRANSFER PROTEIN FROM RICE, AK104879

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2004-12-16
Detector	APS-1
Spacegroup name	P 43 21 2
Unit cell lengths	100.558, 100.558, 69.568
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.770 - 2.000
R-factor	0.17525
Rwork	0.172
R-free	0.22720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1wn0
RMSD bond length	0.020
RMSD bond angle	1.609
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (refmac_5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.770	2.050
High resolution limit [Å]	2.000	2.000
Rmerge	0.105	0.596
Number of reflections	24495
<I/σ(I)>	16.747	3.521
Completeness [%]	98.9	99.5
Redundancy	8.4	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5	293	10 MG/ML PROTEIN, 1.0 M SODIUM CITRATE, 0.10 M PIPES, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K, pH 6.50