1YVE
ACETOHYDROXY ACID ISOMEROREDUCTASE COMPLEXED WITH NADPH, MAGNESIUM AND INHIBITOR IPOHA (N-HYDROXY-N-ISOPROPYLOXAMATE)
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-07-12 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 111.430, 61.940, 162.540 |
| Unit cell angles | 90.00, 95.08, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.650 |
| R-factor | 0.197 |
| Rwork | 0.197 |
| R-free | 0.23900 |
| RMSD bond length | 0.014 * |
| RMSD bond angle | 1.710 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Rmerge | 0.064 |
| Number of reflections | 242843 |
| Completeness [%] | 92.0 |
| Redundancy | 3.99 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 7.2 * | 20 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | ammonium sulfate | 1.8 (M) | |
| 2 | 1 | 1 | Tris-HCl | 0.1 (M) | |
| 3 | 1 | 1 | IpOHA | ||
| 4 | 1 | 1 | NADPH | 10 ligand molecules/enzyme monomer | |
| 5 | 1 | 1 | 100 ions/enzyme monomer |
