1YVB
the Plasmodium falciparum Cysteine Protease Falcipain-2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2004-02-08 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.115875 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 96.036, 96.036, 124.164 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 - 2.700 |
R-factor | 0.2322 |
Rwork | 0.232 |
R-free | 0.26410 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1atk |
RMSD bond length | 0.019 |
RMSD bond angle | 1.500 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.000 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.148 | 0.423 |
Number of reflections | 16071 | 1426 |
<I/σ(I)> | 8.9 | 2 |
Completeness [%] | 96.5 | 85 |
Redundancy | 6 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 277 | 0.1 M HEPES pH 7.5 and 4.3 M NaCl , pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |