1YV7
X-ray structure of (C87S,des103-104) onconase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS |
| Temperature [K] | 298 |
| Detector technology | AREA DETECTOR |
| Collection date | 2003-03-19 |
| Detector | ENRAF-NONIUS |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 32.507, 41.265, 69.605 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| R-factor | 0.15 |
| Rwork | 0.145 |
| R-free | 0.19400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1onc |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.066 | 0.177 |
| Number of reflections | 7322 | |
| Completeness [%] | 93.5 | 92.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 277 | PEG 8000, lithium sulphate, sodium acetate, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
