1YV2
Hepatitis C virus NS5B RNA-dependent RNA Polymerase genotype 2a
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.115870 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 60.765, 215.037, 124.139 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.620 - 2.500 |
| R-factor | 0.201 |
| Rwork | 0.201 |
| R-free | 0.25200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 27506 | |
| <I/σ(I)> | 9.4 | 2.9 |
| Completeness [%] | 96.9 | 97.8 |
| Redundancy | 3.6 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 15% PEG 8000, 0.2M ammonium sulphate, 80mM sodium citrate pH 6.0, 7% glycerol, 4% 1,6 hexanediol and 1% benzamidine, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
