1YUW

crystal structure of bovine hsc70(aa1-554)E213A/D214A mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-D
Temperature [K]	113
Detector technology	IMAGE PLATE
Collection date	2004-12-14
Detector	RIGAKU RAXIS HTC
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	65.694, 50.140, 87.156
Unit cell angles	90.00, 99.86, 90.00

Refinement procedure

Resolution	50.000 - 2.600
Rwork	0.220
R-free	0.29700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.570
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.690
High resolution limit [Å]	2.600	2.600
Rmerge	0.079	0.459
Number of reflections	16521
Completeness [%]	94.3	97.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	8	289	PEG8000,trimethyl amine oxide, pH 8.0, microbatch, temperature 289K