1YUW
crystal structure of bovine hsc70(aa1-554)E213A/D214A mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-D |
Temperature [K] | 113 |
Detector technology | IMAGE PLATE |
Collection date | 2004-12-14 |
Detector | RIGAKU RAXIS HTC |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 65.694, 50.140, 87.156 |
Unit cell angles | 90.00, 99.86, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.600 |
Rwork | 0.220 |
R-free | 0.29700 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.570 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.690 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.079 | 0.459 |
Number of reflections | 16521 | |
Completeness [%] | 94.3 | 97.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 8 | 289 | PEG8000,trimethyl amine oxide, pH 8.0, microbatch, temperature 289K |