1YUL
Crystal Structure of Nicotinic Acid Mononucleotide Adenylyltransferase from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-18B |
| Synchrotron site | Photon Factory |
| Beamline | BL-18B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-11-28 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9791, 0.9793, 0.9500 |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 65.525, 65.525, 110.340 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 9.970 - 2.000 |
| R-factor | 0.202 |
| Rwork | 0.202 |
| R-free | 0.24800 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.073 | 0.325 |
| Number of reflections | 31029 | |
| <I/σ(I)> | 11.5 | 11.5 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 7.6 | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | bis-tris propane, trisodium citrate, glycerol , pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
