1YUE

Bacteriophage T4 capsid vertex protein gp24

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	100
Detector technology	CCD
Collection date	2004-06-24
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9
Spacegroup name	P 65
Unit cell lengths	107.614, 107.614, 82.636
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.000 - 2.900
Rwork	0.270
R-free	0.30000
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.460
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	35.000
High resolution limit [Å]	2.900
Rmerge	0.073
Number of reflections	11621
<I/σ(I)>	24.6
Completeness [%]	97.7
Redundancy	14.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.1 M HEPES-Na pH 7.5; 10% v/v isopropanol; 0.2 M Sodium Citrate, VAPOR DIFFUSION, SITTING DROP, temperature 293K
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.1 M HEPES-Na pH 7.5; 10% v/v isopropanol; 0.2 M Sodium Citrate, VAPOR DIFFUSION, SITTING DROP, temperature 293K