1YU9
GppNHp-Bound Rab4A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-11-23 |
| Detector | MAR scanner 300 mm plate |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 68.808, 68.808, 90.920 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 7.000 - 2.070 |
| R-factor | 0.1582 |
| Rwork | 0.156 |
| R-free | 0.19547 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Rab3a polyalanine |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.222 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.110 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Number of reflections | 13803 | |
| <I/σ(I)> | 15.1 | 4.1 |
| Completeness [%] | 98.9 | 98.9 |
| Redundancy | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | Ammonium sulfate, MES, Glycerol, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
